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pei max transfection reagent  (Kyfora Bio)


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    Structured Review

    Kyfora Bio pei max transfection reagent
    Pei Max Transfection Reagent, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 99/100, based on 5632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pei/PEI+MAX+Transfection+Reagent/custom%4024765-1%4042438474
    Average 99 stars, based on 5632 article reviews
    pei max transfection reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: CRISPR and compound screens in a novel ex vivo tissue model identify DDR1 and ETA as regulators of cancer cell invasion.
    Article Snippet: .. G plasmids supplemented with 30 μl of PEI (Cat. 23966, Polysciences, Warrington, USA) were mixed with 500 μl of DMEM without FCS on the day of transfection. ..

    Article Title: SEL1L3 suppresses colorectal cancer cell growth and metastasis by preventing endoplasmic reticulum-associated degradation of STING.
    Article Snippet: .. Transient transfections were carried out using PEI (23966-2, Polysciences) or Lipofectamine 3000 reagent (L3000015, Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: Targeting IRF1-TRIM21 axis enhances anti-tumor immunity by promoting ubiquitin-mediated degradation of FGL1 in non-small cell lung cancer.
    Article Snippet: .. Once the cells reached approximately 90% confluency, we transfected them using PEI (Polysciences, 23966). ..

    Article Title: Structural Insights into Tanc1/2 Autoinhibition and Their Implications for NDD Pathogenesis.
    Article Snippet: Tanc1 and Tanc2 are synaptic scaffold proteins with high structural similarity but distinct expression patterns and functions.. Although accumulating evidence points to important divergent roles of Tanc1 and Tanc2 in neural development, the precise molecular mechanisms governing their activity (e.g., autoinhibition and activation) and their pathogenic pathways in neurodevelopmental disorders remain poorly defined.. Here, we investigated the molecular basis underlying mouse Tanc1 and Tanc2 autoinhibition, ATP-dependent activation, and disease-mutation induced hyperactivation.

    Article Title: Exploration of targeted electrophilic kinase probes identifies a covalent ULK1 degrader
    Article Snippet: Briefly, Flag-ULK1 expressing stable SH-SY5Y cell lines were generated as follows: HEK293FT cells were used to produce retroviruses and co-transfected with 3.8 μg pCMV-gag-pol (Cell Biolabs; cat no: #RV-111), 2.2 μg pCMV-VSV-G (Cell Biolabs; Cat no: #RV-110) and 6 μg of pBabeD-Flag-ULK1 WT (MRC Reagents and Services; DU83134). .. Plasmids were added into 600 μl Opti-MEM (Gibco) and 24 μl of PEI (1 mg/ml, Polysciences, #24765) dissolved in 25 mM HEPES buffer (pH 7.5) and vortexed for 15 s and transfection mixture incubated for 20 min at room temperature and added dropwise onto cells that were plated in 10 cm plates 16 h prior to transfection. ..

    Article Title: In vivo CRISPR-based screen identifies ZC3H12C as a mediator of CAR-T cell dysfunction in solid tumors
    Article Snippet: .. Eighteen hours later, cells were transfected with 18 μg of transfer vector (containing CAR) and a pre-mixed packaging mix containing 15 μg of pREV, 15 μg of pRRE and 7 μg of pVSV, using PEI (Polysciences, #23966-100). .. The lentivirus-containing supernatant was collected 48 and 72h after transfection, 0.45 μm filtered, and concentrated using Lenti-X Concentrator (TakaraBio, #631232) as per manufacturer’s instructions.

    Expressing:

    Article Title: Structural Insights into Tanc1/2 Autoinhibition and Their Implications for NDD Pathogenesis.
    Article Snippet: Tanc1 and Tanc2 are synaptic scaffold proteins with high structural similarity but distinct expression patterns and functions.. Although accumulating evidence points to important divergent roles of Tanc1 and Tanc2 in neural development, the precise molecular mechanisms governing their activity (e.g., autoinhibition and activation) and their pathogenic pathways in neurodevelopmental disorders remain poorly defined.. Here, we investigated the molecular basis underlying mouse Tanc1 and Tanc2 autoinhibition, ATP-dependent activation, and disease-mutation induced hyperactivation.

    Plasmid Preparation:

    Article Title: Structural Insights into Tanc1/2 Autoinhibition and Their Implications for NDD Pathogenesis.
    Article Snippet: Tanc1 and Tanc2 are synaptic scaffold proteins with high structural similarity but distinct expression patterns and functions.. Although accumulating evidence points to important divergent roles of Tanc1 and Tanc2 in neural development, the precise molecular mechanisms governing their activity (e.g., autoinhibition and activation) and their pathogenic pathways in neurodevelopmental disorders remain poorly defined.. Here, we investigated the molecular basis underlying mouse Tanc1 and Tanc2 autoinhibition, ATP-dependent activation, and disease-mutation induced hyperactivation.

    Concentration Assay:

    Article Title: Structural Insights into Tanc1/2 Autoinhibition and Their Implications for NDD Pathogenesis.
    Article Snippet: Tanc1 and Tanc2 are synaptic scaffold proteins with high structural similarity but distinct expression patterns and functions.. Although accumulating evidence points to important divergent roles of Tanc1 and Tanc2 in neural development, the precise molecular mechanisms governing their activity (e.g., autoinhibition and activation) and their pathogenic pathways in neurodevelopmental disorders remain poorly defined.. Here, we investigated the molecular basis underlying mouse Tanc1 and Tanc2 autoinhibition, ATP-dependent activation, and disease-mutation induced hyperactivation.

    Incubation:

    Article Title: Exploration of targeted electrophilic kinase probes identifies a covalent ULK1 degrader
    Article Snippet: Briefly, Flag-ULK1 expressing stable SH-SY5Y cell lines were generated as follows: HEK293FT cells were used to produce retroviruses and co-transfected with 3.8 μg pCMV-gag-pol (Cell Biolabs; cat no: #RV-111), 2.2 μg pCMV-VSV-G (Cell Biolabs; Cat no: #RV-110) and 6 μg of pBabeD-Flag-ULK1 WT (MRC Reagents and Services; DU83134). .. Plasmids were added into 600 μl Opti-MEM (Gibco) and 24 μl of PEI (1 mg/ml, Polysciences, #24765) dissolved in 25 mM HEPES buffer (pH 7.5) and vortexed for 15 s and transfection mixture incubated for 20 min at room temperature and added dropwise onto cells that were plated in 10 cm plates 16 h prior to transfection. ..



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